Chinese Optics Letters, Vol. 18, Issue 12, 121701 (2020)
Optical-resolution photoacoustic microscopy continually monitors macrophages activities of acute inflammation in vivo
Fei Duan1, Haosong Ma1, Jinde Zhang1, Shi Li1, Honghui Li1, Zhiyou Wu1, Fengqiu Hong1, Lüming Zeng2,3, and Liming Nie1,*
- 1State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics & Center for Molecular Imaging and Translational Medicine, School of Public Health, Xiamen University, Xiamen 361102, China
- 2State Key Laboratory of Precision Electronic Manufacturing Technology and Equipment, School of Electromechanical Engineering, Guangdong University of Technology, Guangzhou 510006, China
- 3Jiangxi Key Laboratory of Optic-Electronic and Communication, Jiangxi Science and Technology Normal University, Nanchang 330038, China
Photoacoustic imaging has been developed to image the immune study at the macro scale. Macrophages play diverse roles in the acute response to infection and tissue repair. However, macrophages activities in acute inflammation at the microscopic level still remain challenging. In this work, we proposed optical-resolution photoacoustic microscopy to promptly monitor the labeled macrophages activities in normal and inflammatory groups. The result showed that many labeled macrophages emerged around the vessels firstly, then exuded into tissues, and finally disappeared in the inflammatory group injected with labeled macrophages. In summary, our method allows us to exactly image and track the immune cells of inflammatory diseases.
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