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Main > Chinese Optics Letters >  Volume 19 >  Issue 4 >  Page 041101 > Article
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    • Received: Jun. 1, 2020

    Accepted: Oct. 9, 2020

    Posted: Dec. 28, 2020

    Published Online: Jan. 8, 2021

    The Author Email: Xinwei Wang (wangxinwei@semi.ac.cn)

    DOI: 10.3788/COL202119.041101

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    Xin Zhong, Xinwei Wang, Liang Sun, Yan Zhou. Enhancement of rapid lifetime determination for time-resolved fluorescence imaging in forensic examination[J]. Chinese Optics Letters, 2021, 19(4): 041101

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Chinese Optics Letters, Vol. 19, Issue 4, 041101 (2021)

Enhancement of rapid lifetime determination for time-resolved fluorescence imaging in forensic examination

Xin Zhong1,2, Xinwei Wang1,2,3,*, Liang Sun1, and Yan Zhou1,2,3

Author Affiliations

  • 1Optoelectronics System Laboratory, Institute of Semiconductors, Chinese Academy of Sciences, Beijing 100083, China
  • 2College of Materials Science and Opto-Electronics Technology, University of Chinese Academy of Sciences, Beijing 100049, China
  • 3School of Electronic, Electrical and Communication Engineering, University of Chinese Academy of Sciences, Beijing 100049, China

Abstract

An enhancement method of rapid lifetime determination is proposed for time-resolved fluorescence imaging to discriminate substances with approximate fluorescence lifetime in forensic examination. In the method, an image-exclusive-OR treatment with filter threshold adaptively chosen is presented to extract the region of interest from dual-gated fluorescence intensity images, and then the fluorescence lifetime image is reconstructed based on the rapid lifetime determination algorithm. Furthermore, a maximum and minimum threshold filtering is developed to automatically realize visualization enhancement of the lifetime image. In proof experiments, compared with traditional fluorescence intensity imaging and rapid lifetime determination method, the proposed method automatically distinguishes altered and obliterated documents written by two brands of highlighters with the same color and close fluorescence lifetime.

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