Chinese Journal of Lasers, Vol. 46, Issue 9, 0907001 (2019)
High-Resolution Photoacoustic Quantitative Imaging of Tumor Vessels
Chen Ningbo1,2, Zhou Huichao2,3, Zhao Huangxuan2, Wang Boquan1,2, Song Liang2, Liu Chengbo2,**, and Zhang Jianhui1,*
- 1 School of Mechanical and Electrical Engineering, Guangzhou University, Guangzhou, Guangdong 510006, China
- 2 Research Lab for Biomedical Optics and Molecular Imaging, Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences, Shenzhen, Guangdong 518055, China
- 3 Department of Ultrasound, the Third Affiliated Hospital, Sun Yat-Sen University,Guangzhou, Guangdong 510630, China
We uses a high-resolution (micrometer scale) photoacoustic microscopic imaging system to continuously monitor early-stage tumor angiogenesis in the ear of mice and its response to anti-angiogenesis therapy. Further, a three-dimensional Hessian-matrix-based vascular extraction algorithm is proposed for the quantitative photoacoustic imaging of tumor vessels to improve the tumor vessel extraction accuracy. Subsequently, the morphological changes in various parameters, such as the diameter, density, and tortuosity, of the tumor vessels are quantitatively analyzed. Furthermore, the potential of high-resolution photoacoustic quantitative imaging to study the pathological mechanisms of tumors and other diseases characterized by vascular changes is demonstrated in this study.