Advanced Photonics, Vol. 1, Issue 5, 055001 (2019)
Robust and rapidly tunable light source for SRS/CARS microscopy with low-intensity noise
Heiko Linnenbank1,2,*, Tobias Steinle1,2, Florian Mörz1,2, Moritz Flöss2, Han Cui3, Andrew Glidle3, and Harald Giessen1,2
- 1SI Stuttgart Instruments GmbH, Öhringen, Germany
- 2University of Stuttgart, 4th Physics Institute and Research Center SCoPE, Stuttgart, Germany
- 3University of Glasgow, College of Science and Engineering, Division of Biomedical Engineering, Glasgow, United Kingdom
We present a fully automated laser system with low-intensity noise for coherent Raman scattering microscopy. The robust two-color system is pumped by a solid-state oscillator, which provides Stokes pulses fixed at 1043 nm. The tunable pump pulses of 750 to 950 nm are generated by a frequency-doubled fiber-feedback femtosecond optical parametric oscillator. The resulting pulse duration of 1.2 ps provides a viable compromise between optimal coherent Raman scattering signal and the necessary spectral resolution. Thus a spectral range of 1015 to 3695 cm−1 with spectral resolution of <13 cm−1 can be addressed.