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  • Received: Jul. 4, 2019

    Accepted: Aug. 28, 2019

    Posted: Nov. 2, 2019

    Published Online: Sep. 25, 2019

    The Author Email: Linnenbank Heiko (h.linnenbank@pi4.uni-stuttgart.de)

    DOI: 10.1117/1.AP.1.5.055001

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    Heiko Linnenbank, Tobias Steinle, Florian Mörz, Moritz Flöss, Han Cui, Andrew Glidle, Harald Giessen. Robust and rapidly tunable light source for SRS/CARS microscopy with low-intensity noise[J]. Advanced Photonics, 2019, 1(5): 055001

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Advanced Photonics, Vol. 1, Issue 5, 055001 (2019)

Robust and rapidly tunable light source for SRS/CARS microscopy with low-intensity noise 

Heiko Linnenbank1,2,*, Tobias Steinle1,2, Florian Mörz1,2, Moritz Flöss2, Han Cui3, Andrew Glidle3, and Harald Giessen1,2

Author Affiliations

  • 1SI Stuttgart Instruments GmbH, Öhringen, Germany
  • 2University of Stuttgart, 4th Physics Institute and Research Center SCoPE, Stuttgart, Germany
  • 3University of Glasgow, College of Science and Engineering, Division of Biomedical Engineering, Glasgow, United Kingdom

Abstract

We present a fully automated laser system with low-intensity noise for coherent Raman scattering microscopy. The robust two-color system is pumped by a solid-state oscillator, which provides Stokes pulses fixed at 1043 nm. The tunable pump pulses of 750 to 950 nm are generated by a frequency-doubled fiber-feedback femtosecond optical parametric oscillator. The resulting pulse duration of 1.2 ps provides a viable compromise between optimal coherent Raman scattering signal and the necessary spectral resolution. Thus a spectral range of 1015 to 3695 cm−1 with spectral resolution of <13 cm−1 can be addressed.

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