Acta Optica Sinica, Vol. 40, Issue 24, 2412001 (2020)
Adaptive Method of Excitation Light Intensity Based on TPLIF Technology for Fast and Accurate Measurement of Photosynthetic Fluorescence Parameters
Wang Xiang1,2, Ying Gaofang1,*, Zhao Nanjing1, Gan Tingting1, Qin Zhisong1,3, Chen Min1,2, Hua Hui1,2, Dong Ming1,2, Ding Zhichao1,2, Qi Peilong1,2, Wang Lu1,2, Meng Deshuo1, and Liu Jianguo1
- 1Key Laboratory of Environment Optics and Technology, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei, Auhui 230031, China
- 2University of Science and Technology of China, Hefei, Auhui 230026, China
- 3School of Computer and Information Security, Guilin University of Electronic Technology, Guilin, Guangxi 541004, China
Based on the model of algae fluorescence yield and with the fluorescence saturation parameter Eσ as the judgment standard of saturated excitation light intensity, we proposed a self-adaptive method of excitation light intensity to accurately acquire the photosynthetic fluorescence parameters of different species of algae. The results show that with the self-adaptive method, the saturated excitation light intensity could be quickly and stably obtained in the seven species of algae, and the relative standard deviations (RSDs) of the adjustment results were all smaller than 2.5%. Furthermore, the effective light absorption cross-sections σPSII of the seven species of algae were measured by the tunable pulse laser induced fluorescence (TPLIF) technique based on the self-adaptive method, and the difference in the σPSII of different species of algae was analyzed. Finally, we measured the Chlorella samples at different growth stages through the TPLIF technique and recorded their fluorescence parameter Fv/Fm and relevant variation trend. It turns out that the recorded results are consistent with the measurement results of the photosynthetic activity analyzer Fast-Ocean, with the correlation coefficient being 0.9939. In conclusion, this study provides an effective way of saturation excitation for accurately measuring the photosynthetic fluorescence parameters of different species of algae at different growth stages (with obvious differences in growth status).
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