Received: Mar. 15, 2018
Accepted: Aug. 13, 2018
Published Online: Dec. 26, 2018
The Author Email: Wang Wei (firstname.lastname@example.org)
Xuan Chu, Wei Wang, Xinzhi Ni, Haitao Zheng, Xin Zhao, Hong Zhuang, Kurt C., Chunyang Li, Yufeng Li, Chengjun Lu. Evaluation of growth characteristics of Aspergillus parasiticus inoculated in different culture media by shortwave infrared (SWIR) hyperspectral imaging[J]. Journal of Innovative Optical Health, 2018, 11(5):1850031
Xuan Chu1,2, Wei Wang1,*, Xinzhi Ni3, Haitao Zheng4, Xin Zhao1, Hong Zhuang5, Kurt C.6, Chunyang Li7, Yufeng Li8, and Chengjun Lu9
The growth characteristics of Aspergillus parasiticus incubated on two culture media were examined using shortwave infrared (SWIR, 1000–2500 nm) hyperspectral imaging (HSI) in this work. HSI images of the A. parasiticus colonies growing on rose bengal medium (RBM) and maize agar medium (MAM) were recorded daily for 6 days. The growth phases of A. parasiticus were indicated through the pixel number and average spectra of colonies. On score plot of the first principal component (PC1 T and PC2, four growth zones with varying mycelium densities were identified. Eight characteristic wavelengths (1095, 1145, 1195, 1279, 1442, 1655, 1834 and 1929 nm) were selected from PC1 loading, average spectra of each colony as well as each growth zone. Furthermore, support vector machine (SVM) classifier based on the eight wavelengths was built, and the classification accuracies for the four zones (from outer to inner zones) on the colonies on RBM were 99.77%, 99.35%, 99.75% and 99.60% and 99.77%, 99.39%, 99.31% and 98.22% for colonies on MAM. In addition, a new score plot of PC2 and PC3 was used to differentiate the colonies incubated on RBM and MAM for 6 days. Then characteristic wavelengths of 1067, 1195, 1279, 1369, 1459, 1694, 1834 and 1929 nm were selected from the loading of PC2 and PC3. Based on them, a new SVM model was developed to differentiate colonies on RBM and MAM with accuracy of 100.00% and 99.99%, respectively. In conclusion, SWIR hyperspectral image is a powerful tool for evaluation of growth characteristics of A. parasiticus incubated in different culture media.