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  • Received: Jun. 28, 2020

    Accepted: Aug. 11, 2020

    Posted: Sep. 28, 2020

    Published Online: Sep. 29, 2020

    The Author Email: Yasuyuki Ozeki (ozeki@ee.t.u-tokyo.ac.jp)

    DOI: 10.3788/COL202018.121702

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    Yasuyuki Ozeki. Molecular vibrational imaging by stimulated Raman scattering microscopy: principles and applications [Invited][J]. Chinese Optics Letters, 2020, 18(12): 121702

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Schematics and optical spectra of (a), (b) spontaneous Raman scattering and (c), (d) CRS. Adapted and modified from Ref. [3].

Fig. 1. Schematics and optical spectra of (a), (b) spontaneous Raman scattering and (c), (d) CRS. Adapted and modified from Ref. [3].

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Typical schematic of SRS microscope. PD, photodiode. Adapted and modified from Ref. [3].

Fig. 2. Typical schematic of SRS microscope. PD, photodiode. Adapted and modified from Ref. [3].

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Multicolor SRS methods based on (a) wavelength scanning, (b) spectral focusing, (c) wavelength-multiplexing, and (d) modulation-frequency multiplexing. Adapted from Ref. [3].

Fig. 3. Multicolor SRS methods based on (a) wavelength scanning, (b) spectral focusing, (c) wavelength-multiplexing, and (d) modulation-frequency multiplexing. Adapted from Ref. [3].

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Examples of SRS imaging reported by the author and co-workers. (a) Hyperspectral SRS image of two types of polymer beads [polystyrene and poly(methyl methacrylate) (PMMA)]. (b) SRS spectra taken at the arrows shown in (a)[12]. (c) Hyperspectral SRS image of acetaminophen-overdosed mouse liver tissue[30]. (d), (e) Uptake of boron cluster compound cholesterol by HeLa cells for (d) 2 h and (e) 4 h[39]. (f) Multicolor SRS imaging flow cytometry of Euglena gracilis cells cultured in different conditions. Green, chlorophyll; red, paramylon; blue, lipid[40].

Fig. 4. Examples of SRS imaging reported by the author and co-workers. (a) Hyperspectral SRS image of two types of polymer beads [polystyrene and poly(methyl methacrylate) (PMMA)]. (b) SRS spectra taken at the arrows shown in (a)[12]. (c) Hyperspectral SRS image of acetaminophen-overdosed mouse liver tissue[30]. (d), (e) Uptake of boron cluster compound cholesterol by HeLa cells for (d) 2 h and (e) 4 h[39]. (f) Multicolor SRS imaging flow cytometry of Euglena gracilis cells cultured in different conditions. Green, chlorophyll; red, paramylon; blue, lipid[40].

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