Chinese Optics Letters, Vol. 18, Issue 7, 071701 (2020)
Three-dimensional tracking of multiple particles in large depth of field using dual-objective bifocal plane imaging
Aiwang Huang1,2,3, Danni Chen1,2,3,*, Heng Li1,2,3, Dexiang Tang1,2,3, Bin Yu1,2,3, Jia Li1,2,3, and Junle Qu1,2,3
- 1Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China
- 2Shenzhen Key Laboratory of Biomedicine Engineering, Shenzhen University, Shenzhen 518060, China
- 3Key Laboratory of Micro-Nano Measuring and Imaging in Biomedical Optics, Shenzhen University, Shenzhen 518060, China
Tracking moving particles in cells by single particle tracking is an important optical approach widely used in biological research. In order to track multiple particles within a whole cell simultaneously, a parallel tracking approach with large depth of field was put forward. It was based on distorted grating and dual-objective bifocal imaging, making use of the distorted grating to expand the depth of field, dual-objective to gather as many photons as possible, and bifocal plane imaging to realize three-dimensional localization. Simulation of parallel tracking of two particles moving along the z axis demonstrated that even when the two are axially separated by 10 μm, they can both be localized simultaneously with transversal precision better than 5 nm and axial precision better than 20 nm.
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