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  • Received: May. 20, 2020

    Accepted: Aug. 11, 2020

    Posted: Sep. 30, 2020

    Published Online: Oct. 9, 2020

    The Author Email: Fake Lu (fakelu@binghamton.edu)

    DOI: 10.3788/COL202018.121703

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    Fangyu Wang, Yuhao Yuan, Qiang Sun, Ming Dai, Li Ai, Fake Lu. Design and implementation of the galvanometer scanning system for reflectance confocal and stimulated Raman scattering microscopy[J]. Chinese Optics Letters, 2020, 18(12): 121703

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Chinese Optics Letters, Vol. 18, Issue 12, 121703 (2020)

Design and implementation of the galvanometer scanning system for reflectance confocal and stimulated Raman scattering microscopy

Fangyu Wang1,2,3,4, Yuhao Yuan4, Qiang Sun1, Ming Dai1, Li Ai5,6, and Fake Lu4,*

Author Affiliations

  • 1Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun 130033, China
  • 2University of Chinese Academy of Sciences, Beijing 100049, China
  • 3Jilin Yataizhongke Medical Equipment Engineering Technology Research Institute Holding Co., Ltd., Changchun 130000, China
  • 4Department of Biomedical Engineering, Binghamton University, State University of New York, Binghamton, NY 13902, USA
  • 5Jilin Provincial Key Laboratory of Photoelectric Equipment and Instrument Advanced Manufacture Technology, Changchun 130033, China
  • 6Changchun UP Optotech Holding Co., Ltd., Changchun 130033, China

Abstract

We report on two strategies to design and implement the galvanometer-based laser-scanning mechanisms for the realization of reflectance confocal microscopy (RCM) and stimulated Raman scattering (SRS) microscopy systems. The RCM system uses a resonant galvanometer scanner driven by a home-built field-programmable gate array circuit with a novel dual-trigger mode and a home-built high-speed data acquisition card. The SRS system uses linear galvanometers with commercially available modules. We demonstrate video-rate high-resolution imaging at 11 frames per second of in vivo human skin with the RCM system and label-free biomolecular imaging of cancer cells with the SRS system. A comparison of the two strategies for controlling galvanometer scanners provides scientific and technical reference for future design and commercialization of various laser-scanning microscopes using galvanometers.

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