Chinese Optics Letters, 2020, Vol. 18, Issue 10, pp.
Tomography-assisted Doppler photoacoustic microscopy: proof of concept [Early Posting]
Wang Xinkun, Xiong Kedi, Jin Xin, Yang Sihua
The previous methods to measure flow speeds by photoacoustic microscopy solely focused on either the transverse or the axial flow component, which did not reflect absolute flow speed. Here we present absolute flow speed maps by combining Doppler bandwidth broadening with volumetric photoacoustic microscopy. Photoacoustic Doppler bandwidth broadening and photoacoustic tomographic images were applied to measure the transverse flow component and the Doppler angle, respectively. Phantom experiments quantitatively demonstrated that a range of 55º to 90º Doppler angle, and 0.5 to 10 mm/s of flow speed can be measured. This tomography-assisted method provides the foundation for further measurement in vivo.